Direct proof of use
Afghan National Army Air Corps Mi-35 Hind E helicopters were documented in combat use during the 2001 War in Afghanistan. U.S. Air Force reporting in May 2010 said Afghan Mi-35 aircrews had been employed against Taliban forces in Baghlan Province and had also provided armed escort for Mi-17 helicopters carrying Afghan National Army soldiers to a forward location.
The same U.S. Air Force account placed the Mi-35 program inside a joint advisory effort by American, Czech, and Hungarian personnel assigned to the 440th Air Expeditionary Advisory Squadron and related mentor teams. The reported mission set included weapons firing, command and control, forward-observer coordination, presidential air-squadron escort operations, and continuing kinetic operations.
Sources: Airman leads joint Mi-35 attack helicopter adviser team for Afghan Air Corps
Dated combat milestones
Open reporting gives two dated 2010 combat examples. Long War Journal reported that on April 20, Afghan Mi-35s were called to Kunduz to support the Afghan National Army's 209th Corps during an engagement with a large Taliban force, and that on May 12 several Hinds engaged armed men running a roadblock outside Kabul.
Those reported examples match the broader U.S. Air Force account that Afghan Mi-35 crews had moved from range work and advisory training into kinetic and armed-escort missions by May 2010. The sources do not establish a complete sortie history for the aircraft; they document specific missions and the operational role that Afghan crews were being prepared to sustain.
Sources: Soviet workhorses, ISAF training form the backbone of a developing Afghan Air Force, Airman leads joint Mi-35 attack helicopter adviser team for Afghan Air Corps