Direct proof of use
The AGM-86C Conventional Air-Launched Cruise Missile is directly documented in the 1990 Gulf War through U.S. Air Force accounts of Operation Desert Storm. The Air Force fact sheet says the CALCM became operational in January 1991 and that seven B-52s from Barksdale Air Force Base launched 35 missiles from designated points in U.S. Central Command's area of responsibility to attack high-priority targets in Iraq.
Barksdale Air Force Base identifies the opening mission as Operation Senior Surprise, known by crews as Secret Squirrel, and describes seven B-52G Stratofortresses from the 2nd Bomb Wing flying from Barksdale at the start of Operation Desert Storm. Its account says the mission used GPS-guided conventional air-launched cruise missiles against combat targets tied to Iraqi communications infrastructure.
Sources: AGM-86B/C/D Missiles | U.S. Air Force, Barksdale Desert Storm 2nd Bomb Wing
Timeline
The documented use occurred at the opening of the coalition air campaign in January 1991. The Air Force fact sheet places CALCM operational entry at the onset of Operation Desert Storm, while Barksdale dates the 2nd Bomb Wing launch to the early morning of January 16, 1991.
The mission was a long-range round trip from Barksdale rather than a forward-based launch. Air Force and Barksdale accounts describe the sorties as more than 14,000 miles or nautical miles and about 35 hours, making them the longest known combat aircraft sorties up to that point.
Sources: AGM-86B/C/D Missiles | U.S. Air Force, Barksdale Desert Storm 2nd Bomb Wing
Operational role
In the 1990 Gulf War, the AGM-86C's documented role was long-range conventional standoff strike. It allowed B-52 crews to launch GPS-aided cruise missiles from outside the immediate target area against fixed, high-priority Iraqi targets during the first phase of the air campaign.
The available official accounts identify Barksdale B-52 crews, 35 AGM-86C launches, Iraqi high-priority targets, and communications or command-related infrastructure as the mission context. They do not provide a public missile-by-missile target list or battle-damage assessment for each CALCM.
Sources: AGM-86B/C/D Missiles | U.S. Air Force, Barksdale Desert Storm 2nd Bomb Wing, CSIS AGM-86 Air-Launched Cruise Missile
Variant context
The conflict-use claim concerns the AGM-86C CALCM, not the nuclear AGM-86B role. The Air Force says the C model came from AGM-86B missiles retrofitted with a high-explosive blast-fragmentation warhead and GPS integrated with inertial navigation, and CSIS separately summarizes the C/D variants as conventional ALCMs whose existence became public after Gulf War use.
Sources: AGM-86B/C/D Missiles | U.S. Air Force, CSIS AGM-86 Air-Launched Cruise Missile