Direct proof of use
Iraq fielded the SA-8 Gecko, the NATO reporting name associated with the 9K33 Osa family, during the 1990 Gulf War. The U.S. Department of Defense's final Gulf War report lists SA-8 missile systems among the organic Army air-defense assets tied into Iraq's broader radar, missile, and antiaircraft network, and the same report records an SA-8 exploding above a Joint Task Force Proven Force KC-135 operating from Incirlik.
Marine Corps University Press describes Iraq's Operation Desert Storm surface-to-air missile arsenal as including the SA-8 Gecko. CSIS's Gulf War chapter gives a more specific order-of-battle estimate, stating that Iraq deployed at least 20 SA-8 batteries with 30 to 40 fire units among its shorter-range surface-to-air missile forces.
Sources: DoD Persian Gulf War Final Report, Marine Corps University Desert War, CSIS Gulf War Air Supremacy
Timeline
Iraq invaded Kuwait on August 2, 1990, and coalition planning subsequently treated Iraqi surface-to-air missile sites, radars, and air-defense command links as major targets for the air campaign. The DoD final report describes Iraq's strategic integrated air-defense system as a priority target set whose control centers, SAM sites, radar sites, and command-and-control nodes had to be reduced before coalition air power could operate freely.
When Operation Desert Storm began on January 17, 1991, coalition suppression of enemy air defenses targeted Iraqi SAM systems, early-warning and tracking radars, mobile launchers, antiaircraft artillery, and the command links that connected them. GAO's later evaluation described the Iraqi IADS as French-supplied and linked to dozens of SAM and antiaircraft artillery sites, but also as vulnerable to rapid degradation under a concerted coalition air campaign.
Sources: DoD Persian Gulf War Final Report, GAO Air Campaign Evaluation
Narrative
The Osa's Gulf War role was defensive rather than offensive: Iraqi forces used SA-8s as part of a layered ground-based air-defense system built from Soviet and Western radars, command links, interceptors, SAMs, and antiaircraft artillery. DoD reporting places SA-8s with organic Army air-defense assets, while CSIS describes them as part of Iraq's shorter-range SAM threat alongside SA-9, SA-13, Roland, and man-portable missile systems.
Coalition air operations suppressed that network through electronic warfare, anti-radiation missiles, strikes on radars and command nodes, and attacks on SAM sites. The DoD report states that early attacks substantially disrupted SAM and AAA radars, enabling coalition aircraft to move to medium and high altitudes; it also notes that low-altitude operations remained exposed to infrared SAMs and antiaircraft fire. CSIS similarly describes Iraq's SA-8 force as large enough to shape coalition countermeasures, but does not establish a specific confirmed aircraft loss caused by an SA-8.
Sources: DoD Persian Gulf War Final Report, CSIS Gulf War Air Supremacy, GAO Air Campaign Evaluation