Direct proof of use
Israeli Air Force commander Amikam Norkin disclosed on 22 May 2018 that Israel had already carried out operational strikes with the F-35. The aircraft in Israeli service is the F-35I Adir, and the disclosure came during Israeli reporting on confrontations with Iranian forces and Syrian air defenses in the Syrian Civil War.
The Times of Israel reported that Norkin said the Israeli Air Force had twice conducted F-35 strikes on two fronts, while also saying those F-35s were not used in the 10 May 2018 strike package against Iranian targets in Syria. NPR's account, citing Haaretz, framed the same disclosure as recent F-35 airstrikes against Iranian targets in Syria and recorded Norkin's description of Syrian surface-to-air missiles fired at Israeli aircraft.
Sources: IAF First F-35 Operational Strike, Israel First F-35 Strikes, NPR F-35 Combat Use in Syria
Timeline
Israel received its first F-35I Adir aircraft in December 2016 and declared the type operational roughly a year later. By May 2018, the Israeli Air Force publicly described the Adir as flying operational missions and said Israel had conducted the first operational F-35 strike in the world.
The 2018 disclosure followed a period of escalating Israeli-Iranian confrontation in Syria, including Iranian rocket fire toward Israeli positions on the Golan Heights, Israeli strikes against Iranian infrastructure in Syria, and Syrian air-defense fire at Israeli aircraft.
Sources: IAF First F-35 Operational Strike, Israel First F-35 Strikes, NPR F-35 Combat Use in Syria
Operational role
The documented role of the F-35I in this conflict was stealth strike aviation in Israel's wider campaign against Iranian military entrenchment and related targets in Syria. Public sources do not identify the exact munitions, mission routes, or individual target coordinates for the two F-35 strike events Norkin referenced.
The reporting links the aircraft to long-range strike activity rather than air defense or close air support in this conflict record. Defense News separately noted that Norkin discussed recent Syrian airstrikes but did not publicly specify where the two F-35 strikes took place, so the page treats the target details as undisclosed.
Sources: Israel First F-35 Strikes, Defense News F-35 Combat Debut, Defense Post F-35 Airstrikes