Direct proof of use
The B-2 Spirit is directly documented in the 2001 War in Afghanistan through the opening air attacks of Operation Enduring Freedom. Whiteman Air Force Base reporting states that the 509th Bomb Wing and its B-2 Spirits led the October 7, 2001 strike against the Taliban regime in Afghanistan and that six B-2s launched during the first three days of the operation.
RAND's study of Operation Enduring Freedom gives the opening-night detail: two Air Force B-2 stealth bombers from Whiteman Air Force Base participated in the October 7 attacks, each carrying 16 2,000-pound GBU-31 JDAMs against Taliban early warning radars and military headquarters buildings. The Council on Foreign Relations similarly lists two B-2s from Whiteman among the aircraft used in the first Afghanistan airstrikes.
Sources: Whiteman Afghanistan Anniversary, RAND Air Power Against Terror, CFR Afghanistan Airstrikes
Timeline
Operation Enduring Freedom began on October 7, 2001, with U.S. and coalition attacks against Taliban and al-Qaeda targets in Afghanistan. The first-night strike package included B-1B and B-52 bombers, naval fighters, cruise missiles, and two B-2s flying from Missouri.
From October 7 to 9, two B-2s took off from Whiteman each night, according to Air & Space Forces Magazine. Whiteman's anniversary account says all six sorties during the first three days exceeded 40 hours, with the longest lasting more than 44 hours and described by a former 509th Bomb Wing commander as a 44.3-hour combat sortie.
Sources: RAND Air Power Against Terror, Air & Space Forces 44 Hours, Whiteman Afghanistan Anniversary
Operational role
The documented user was the U.S. Air Force's 509th Bomb Wing, operating from Whiteman Air Force Base, Missouri. The B-2 role was long-range precision strike at the start of the air campaign, with aerial refueling enabling missions from the continental United States to Afghanistan.
The Air Force's B-2 fact sheet summarizes the Afghanistan use as one of the aircraft's longest missions to date from Whiteman to Afghanistan and back. More detailed accounts describe a westward Pacific route with multiple tanker refuelings, a landing and quick turn at Diego Garcia after the strikes, and return to Whiteman. Public sources identify broad target categories and the opening-night JDAM loadout, but they do not provide a full target list or battle-damage assessment for each B-2 sortie.
Sources: Air Force B-2 Fact Sheet, Air & Space Forces 44 Hours, RAND Air Power Against Terror