Direct proof of use
Afghan Air Force A-29 Super Tucanos are documented in combat use during the 2001 War in Afghanistan. U.S. Air Force reporting said that, after laser-guided bombs were introduced on March 22, 2018, A-29 pilots had supported about 30 Afghan ground missions and dropped more than 50 laser-guided bombs on enemy targets by May 11, 2018.
The clearest single incident was the March 22, 2018 strike in Farah. Military.com reported that an Afghan A-29 squadron used a GBU-58 Paveway II from an A-29 Super Tucano to destroy a Taliban compound, while Army Times identified the pilots as operating from Kabul Air Wing's Kandahar A-29 detachment. A public-domain Resolute Support video page describes the same strike as the first Afghan Air Force combat employment of a laser-guided bomb.
Sources: Laser Guided Bombs enhance Afghan Air Force Strike Capability, Afghan A-29 drops first laser-guided bomb on Taliban, Afghan A-29 pilots drop laser-guided bomb in combat, First laser-guided bomb dropped on Taliban in Farah, Afghanistan (22 March 2018).webm
Timeline
The first four A-29s arrived in Afghanistan on January 15, 2016, when U.S. Air Forces Central described the aircraft as a light air support platform for close air support, aerial escort, armed overwatch, aerial interdiction, and precision-guided munition delivery.
Afghan A-29 airstrike capability followed in 2016, and the laser-guided bomb milestone came on March 22, 2018 in Farah. By May 2018, U.S. Air Force reporting framed the capability as an expanding Afghan-operated strike tool rather than a one-off event, with Afghan ammunition specialists, maintainers, and A-29 pilots supporting the mission set.
Sources: AFCENT Afghan Air Force receives first four A-29 attack aircraft, Laser Guided Bombs enhance Afghan Air Force Strike Capability, Afghan A-29 pilots drop laser-guided bomb in combat